Publications
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Post-incubation pH impacts the lipid extraction assisted by Pulsed Electric Fields (PEF) from wet biomass of Auxenochlorella protothecoides
Delso, C.; Silve, A.; Nazarova, N.; Wüstner, R.; Müller, G.; Frey, W.
2023, September. 16. International Bioelectrics Symposium (BIOELECTRICS 2023), Lisbon, Portugal, September 10–13, 2023 -
Direct transesterification of microalgae after Pulsed Electric Field ( PEF ) treatment
Papachristou, I.; Zhang, S.; Gorte, O.; Ochsenreither, K.; Wüstner, R.; Nazarova, N.; Frey, W.; Silve, A.
2022. Journal of Chemical Technology & Biotechnology, 98 (3), 642–650. doi:10.1002/jctb.7293 -
Einsatz von Elektroimpulsen für die Biotechnologie von Mikroalgen
Frey, W.; Nick, P.
2022. BioSpektrum, 28 (1), 94–96. doi:10.1007/s12268-022-1688-7 -
Conception and Development of a Pulsed Microwave Applicator for Exposure of Fresh Microalgae Biomass
Schlundt, J.; Soldatov, S.; Frey, W.; Link, G.; Baumann, K.; Jelonnek, J.; Sack, M.; Hochberg, M.; Silve, A.
2021. IEEE Transactions on Plasma Science, 49 (9). doi:10.1109/TPS.2021.3101943 -
Biorefinery of Microalgae via Combination of Pulsed Electric Field Treatment and Hydrothermal Liquefaction - A Techno-Economic Assessment
Guo, B.; Frey, W.; Hornung, U.; Dahmen, N.
2020. 28th European Biomass Conference and Exhibition Proceedings, EUBCE 2020, 526–529, ETA-Florence Renewable Energies. doi:10.5071/28thEUBCE2020-3BO.3.4 -
Hydrothermal liquefaction of valuables-extracted microalgae with help of pulsed electric field treatment
Guo, B.; Yang, B.; Silve, A.; Akaberi, S.; Scherer, D.; Papachristou, I.; Frey, W.; Hornung, U.; Dahmen, N.
2019. Proceedings of the 27th European Biomass Conference and Exhibition, 1127–1131, ETA-Florence Renewable Energies. doi:10.5071/27thEUBCE2019-3AV.4.3 -
Hydrothermal liquefaction of valuables-extracted microalgae with help of pulsed electric field treatment
Guo, B.; Yang, B.; Silve, A.; Akaberi, S.; Scherer, D.; Papachristou, I.; Frey, W.; Hornung, U.; Dahmen, N.
2019. 27th European Biomass Conference and Exhibition (EUBCE 2019), Lisbon, Portugal, May 27–30, 2019 -
Renewable Energy (RE): Bioenergy -Feedstocks and Pretreatment-
Akaberi, S.; An, W.; Fetzer, R.; Fleig, J.; Frey, W.; Gusbeth, C.; Heinzel, A.; Herzog, M.; Hochberg, D.; Lang, F.; Leber, K.; Müller, G.; Nazarova, N.; Neukirch, A.; Sack, M.; Scherer, D.; Schumacher, G.; Shi, H.; Silve, A.; Sivkovich, A.; Sträßner, R.; Weisenburger, A.; Wüstner, R.; Zhen, W.
2019. Annual Report 2017 / Institute for Pulsed Power and Microwave Technology / Institut für Hochleistungsimpuls- und Mikrowellentechnik, 21–33, KIT Scientific Publishing. doi:10.5445/IR/1000091706 -
Energy costs of salinity tolerance in crop plants
Tyerman, S. D.; Munns, R.; Fricke, W.; Arsova, B.; Barkla, B. J.; Bose, J.; Bramley, H.; Byrt, C.; Chen, Z.; Colmer, T. D.; Cuin, T.; Day, D. A.; Foster, K.; Gilliham, M.; Henderson, S. W.; Horie, T.; Jenkins, C. L. D.; Kaiser, B. N.; Katsuhara, M.; Plett, D.; Miklavcic, S. J.; Roy, S. J.; Rubio, F.; Shabala, S.; Shelden, M.; Soole, K.; Taylor, N. L.; Tester, M.; Watt, M.; Wege, S.; Wegner, L. H.; Wen, Z.
2019. The new phytologist, 221 (1), 25–29. doi:10.1111/nph.15555 -
Effect of nanosecond pulsed electric field treatment on cell proliferation of microalgae
Buchmann, L.; Frey, W.; Gusbeth, C.; Ravaynia, P. S.; Mathys, A.
2019. Bioresource technology, 271, 402–408. doi:10.1016/j.biortech.2018.09.124 -
Recommendations and requirements for reporting on applications of electric pulse delivery for electroporation of biological samples
Cemazar, M.; Sersa, G.; Frey, W.; Miklavcic, D.; Teissié, J.
2018. Bioelectrochemistry, 122, 69–76. doi:10.1016/j.bioelechem.2018.03.005 -
Incubation time after pulsed electric field treatment of microalgae enhances the efficiency of extraction processes and enables the reduction of specific treatment energy
Silve, A.; Kian, C. B.; Papachristou, I.; Kubisch, C.; Nazarova, N.; Wüstner, R.; Leber, K.; Strässner, R.; Frey, W.
2018. Bioresource technology, 269, 179–187. doi:10.1016/j.biortech.2018.08.060 -
Extraction of lipids from wet microalga Auxenochlorella protothecoides using pulsed electric field treatment and ethanol-hexane blends
Silve, A.; Papachristou, I.; Wüstner, R.; Sträßner, R.; Schirmer, M.; Leber, K.; Guo, B.; Interrante, L.; Posten, C.; Frey, W.
2017. Algal Research, 29, 212–222. doi:10.1016/j.algal.2017.11.016 -
Comparison of extraction of valuable compounds from microalgae by atmospheric pressure plasmas and pulsed electric fields
Zocher, K.; Banaschik, R.; Schulze, C.; Schulz, T.; Kredl, J.; Miron, C.; Schmidt, M.; Mundt, S.; Frey, W.; Kolb, J. F.
2016. Plasma medicine, 6 (3-4), 273–302. doi:10.1615/PlasmaMed.2017019104 -
Microalgae Precipitation in Treatment Chambers during Pulsed Electric Field (PEF) Processing
Strässner, R.; Silve, A.; Eing, C.; Leber, K.; Rocke, S.; Wuestner, R.; Frey, W.
2016. AlgaEurope, Madrid, E, December 13-15, 2016 -
Towards marine biorefineries: Selective proteins extractions from marine macroalgae Ulva with pulsed electric fields
Polikovsky, M.; Fernand, F.; Sack, M.; Frey, W.; Müller, G.; Golberg, A.
2016. Innovative food science & emerging technologies, 37, 194–200. doi:10.1016/j.ifset.2016.03.013 -
Recommendations guidelines on the key information to be reported in studies of application of PEF technology in food and biotechnological processes
Raso, J.; Frey, W.; Ferrari, G.; Pataro, G.; Knorr, D.; Teissie, J.; Miklavcic, D.
2016. Innovative food science & emerging technologies, 37, 312–321. doi:10.1016/j.ifset.2016.08.003 -
Pulsed electric field processing of microalgae. Benefits and limitations
Frey, W.; Gusbeth, C.; Strässner, R.; Silve, A.; Müller, G.
2016. AlgaEurope, Madrid, E, December 13-15, 2016 -
Einsatz der Elektroimpulsbehandlung zur Extraktion von Inhaltsstoffen aus Mikroalgen
Frey, W.; Gusbeth, C.; Silve, A.; Strässner, R.; Müller, G.
2016. 9.Bundesalgenstammtisch, Jülich, 26.-27.September 2016 -
Parasitic Microalgae Precipitation in PEF Treatment Chambers
Straessner, R.; Silve, A.; Frey, W.
2016. 13th International Bioelectrics Symposium, Rostock, September 12-15, 2016 -
Study of transmembrane voltage kinetics during 100μs pulse using voltage sensitive dyes
Silve, A.; Poignard, C.; Sack, M.; Straessner, R.; Frey, W.
2016. 1st World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food and Environmental Technologies (WC 2015), Portoroz, SLO, September 6-10, 2015. Ed.: T. Jarm, 147–150, Springer Science+Business Media. doi:10.1007/978-981-287-817-5_32 -
Impact of external medium conductivity on cell membrane electropermeabilization by microsecond and nanosecond electric pulses
Silve, A.; Leray, I.; Poignard, C.; Mir, L. M.
2016. Scientific reports, 6, Art.Nr.: 19957. doi:10.1038/srep19957 -
Electroporation-based applications in biotechnology
Kotnik, T.; Frey, W.; Sack, M.; Haberl Meglic, S.; Peterka, M.; Miklavcic, D.
2015. Trends in Biotechnology, 33 (8), 480–488. doi:10.1016/j.tibtech.2015.06.002 -
Cell membrane permeabilization by 12-ns electric pulses: Not a purely dielectric, but a charge-dependent phenomenon
Silve, A.; Leray, I.; Leguebe, M.; Poignard, C.; Mir, L. M.
2015. Bioelectrochemistry, 106, 369–378. doi:10.1016/j.bioelechem.2015.06.002 -
Interplay of water and nutrient transport: A whole-plant perspective
Wegner, L. H.
2015. Progress in Botany : Vol.76 Cham : Springer, 2015. Hrsg.: U. Lüttge, 109–141, Springer -
Pulsed power applications in energy research at Karlsruhe Institute of Technology
Müller, G.; Weisenburger, A.; Frey, W.; Sack, M.
2015. 3rd International Scientific Conference ’AtomTech-2015.Electrophysics’, Moskva, Russia, November 17-19, 2015 -
A thermodynamic analysis of the feasibility of water secretion into xylem vessels against a water potential gradient
Wegner, L. H.
2015. Functional plant biology, 42, 828–835. doi:10.1071/FP15077 -
Ressourceneffizienz bei der Verarbeitung von Biomasse mittels Elektroimpulsbehandlung
Frey, W.; Gusbeth, C.
2015. Ressourceneffizienz- und Kreislaufwirtschaftskongress, Stuttgart, 7.-8.Oktober 2015 -
Pulsed electric field processing of microalgae - benefits of cascade processing
Frey, W.; Eing, C.; Gusbeth, C.; Straessner, R.; Silve, A.; Mueller, G.; Schirmer, M.; Posten, C.
2015. ALGAE Europe 2015, Lisboa, P, December 1-3, 2015 -
Pulsed plasma electron source and its application in material surface processing
Müller, G.; Weisenburger, A.; Frey, W.; Sack, M.; Link, G.; Jelonnek, J.
2015. 1st Sino-German Symposium on Atmospheric Pressure Gas Discharges and Plasma Applications, Beijing, China, October 12-16, 2015 -
Electroporation vs. electrostimulation of microalgae: results and heuristic considerations
Gusbeth, C.; Bai, F.; Frey, W.; Nick, P.
2015. 1st World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food and Environmental Technologies, Portoroz, SLO, September 6-10, 2015 Book of Abstracts -
Anwendung der Elektroimpulsbehandlung zur Keimreduktion von Klinik- und kommunalen Abwässern
Gusbeth, C.
2015. Vortr.: Institut für Bioprozess- und Analysenmesstechnik (IBA), Heiligenstadt, 10.Februar 2015 -
Permeabilisation of cells and tissues by electric pulses: Focus on gene electrotransfer
Silve, A.
2015. Vortr.: Aaron Diamond AIDS Research Center, New York, N.Y., 22.Juni 2015 -
Molecular mechanisms of the interaction of electric pulses with cells: a new and unifying model
Breton, M.; Silve, A.; Mir, L. M.
2015. Annual Meeting of Bioelectromagnetics Society European Bioelectromagnetics Association (BioEM 2015), Monterey, Calif., June 14-19, 2015 Book of Abstracts -
Downstream processing of microalgae by pulsed electric fields. Benefits for an energetic use
Frey, W.; Eing, C.; Gusbeth, C.; Straessner, R.
2015. 23rd International Symposium on Bioelectrochemistry and Bioenergetics (BES), Malmö, S, June 14-18, 2015 -
Basic concepts of high voltage pulse generation
Frey, W.
2015. School on Application of Pulsed Electric Fields for Food Processing, Salerno, I, February 7-12, 2015 -
Electroporation of DC-3F cells is a dual process
Wegner, L. H.; Frey, W.; Silve, A.
2015. Biophysical journal, 108, 1660–1671. doi:10.1016/j.bpj.2015.01.038 -
Study of transmembrane voltage kinetics during 100s pulse using voltage sensitive dyes
Silve, A.; Poignard, C.; Sack, M.; Straessner, R.; Frey, W.
2015. 1st World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food and Environmental Technologies, Portoroz, SLO, September 6-10, 2015 Book of Abstracts -
Microalgae precipitation in treatment chambers during PEF processing
Straessner, R.; Eing, C.; Leber, K.; Rocke, S.; Silve, A.; Wüstner, R.; Frey, W.
2015. 1st World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food and Environmental Technologies, Portoroz, SLO, September 6-10, 2015 Book of Abstracts -
Interfacial water probing by CARS spectroscopy on biological samples exposed to intense pulsed electric fields
Azan, A.; Scherman, M.; Silve, A.; Breton, M.; Leray, I.; Dorval, N.; Attal-Treout, B.; Mir, L. M.
2015. 1st World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food and Environmental Technologies, Portoroz, SLO, September 6-10, 2015 Book of Abstracts -
Impact of external medium conductivity on cell membrane electropermeabilization by microsecond and nanosecond electric pulses
Silve, A.; Leray, I.; Poignard, C.; Mir, L. M.
2015. 1st World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food and Environmental Technologies, Portoroz, SLO, September 6-10, 2015 Book of Abstracts -
Dynamic modeling of electroporation for the computation of the electric field distribution inside biological tissues during the application of the pulse voltage
Mir, L. M.; Poignard, C.; Scorretti, R.; Silve, A.; Voyer, D.
2015. 1st World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food and Environmental Technologies, Portoroz, SLO, September 6-10, 2015 Book of Abstracts -
PEF-processing of biomass. Benefits and challenges
Frey, W.; Sack, M.; Eing, C.; Gusbeth, C.; Straessner, R.; Mueller, G.
2015. 1st World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food and Environmental Technologies, Portoroz, SLO, September 6-10, 2015 -
Study of transmembrane voltage dynamics during 100 μs pulses using voltage sensitive dyes
Silve, A.; Rocke, S.; Sack, M.; Frey, W.
2015. From Basics Knowledge to Industry : Electroporation Workshop 2015, Toulouse, F, April 14-15, 2015 -
Pulsed electric field (PEF) processing of microalgae for cell component extraction
Frey, W.; Eing, C.; Gusbeth, C.; Straessner, R.; Posten, C.
2015. Electroporation-based Technologies for Biorefinery Workshop, Compiegne, F, January 27-28, 2015 Book of Abstracts -
PEF assisted extraction of valuable compounds from microalgae C. vulgaris
Pataro, G.; Goettel, M.; Eing, C.; Gusbeth, C.; Straessner, R.; Falcone, M.; Ferraria, G.; Frey, W.
2015. Electroporation-based Technologies for Biorefinery Workshop, Compiegne, F, January 27-28, 2015 Book of Abstracts -
Combined MIPAS (airborne/satellite), CALIPSO and in situ study on large potential NAT particles observed in early Arctic winter stratosphere in December 2011
Woiwode, W.; Höpfner, M.; Pitts, M.; Poole, L.; Oelhaf, H.; Molleker, S.; Borrmann, S.; Ebersoldt, A.; Frey, W.; Gulde, T.; Maucher, G.; Piesch, C.; Sartorius, C.; Orphal, J.
2015. European Geosciences Union (EGU) General Assembly, Wien, A, April 12-17, 2015 Geophysical Research Abstracts, 17(2015) EGU2015-7175 -
Image processing for non-ratiometric measurement of membrane voltage using fluorescent reporters and pulsed laser illumination
Silve, A.; Rocke, S.; Frey, W.
2015. Bioelectrochemistry, 103, 39–43. doi:10.1016/j.bioelechem.2014.07.004 -
The conductance of cellular membranes at supra-physiological voltages
Wegner, L. H.
2015. Bioelectrochemistry, 103, 34–38. doi:10.1016/j.bioelechem.2014.08.005 -
Special Issue for selected papers from Bioelectrics 2013 - 10th International Symposium on Bioelectrics. Co-organized by COST TD1104 "EP4Bio²Med". Editorial
Gilbert, R.; Akiyama, H.; Frey, W.
2015. Bioelectrochemistry, 103(2015). doi:10.1016/j.bioelechem.2015.02.002 -
Numerical models confronted to the ’real life’ of experiments: an overview of the different models, of their strength and of their limits
Poignard, C.; Wegner, L.; Silve, A.
2015. Annual Meeting of Bioelectromagnetics Society European Bioelectromagnetics Association (BioEM 2015), Monterey, Calif., June 14-19, 2015 Book of Abstracts -
Study of transmembrane voltage dynamics during 100μs pulse using voltage sensitive dyes
Silve, A.; Pignard, C.; Leguebe, M.; Rocke, S.; Sack, M.; Frey, W.
2015. Annual Meeting of Bioelectromagnetics Society European Bioelectromagnetics Association (BioEM 2015), Monterey, Calif., June 14-19, 2015 Book of Abstracts